Test Detail

Thrombin-Antithrombin Complex

Test Code:

500012

Performing Lab: LABCORP ESOTERIX COAGULATION LAB

CPT Code:

83520

Order Information
Results

Specimen Container	Blue-top (sodium citrate) tube
Specimen Type	Plasma, FROZEN
Volume	2 mL
Minimum Volume	1 mL
Temperature	FREEZE Stability: Room temperature up to 4 hours Refrigerated up to 4 hours Acceptable freeze/thaw up to 5 cycles
Specimen Handling	Blood should be collected in a blue top tube containing 3.2% buffered sodium citrate.[1] Evacuated collection tube must be filled to completion to ensure a proper blood to anticoagulant ratio.[2,3] The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples.[4,5] When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue top tube. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes. Centrifuge and (using a plastic transfer pipette) carefully remove the plasma without disturbing the cells. Transfer the plasma into a LabCorp PP transpak frozen purple tube with screw cap (LabCorp ID 49482). Freeze immediately and maintain frozen until tested. To avoid delays in turnaround time when requesting multiple test on frozen samples, PLEASE SUBMIT SEPARATE FROZEN SPECIMENS FOR EACH TEST REQUESTED. PRINT AND USE THE SPECIMEN COLLECTION BULLETIN AS A TUBE-FILLING GUIDE.
Methodology	TAT is measured by enzyme immunoassay unitizing a sandwich technique. The patient sample containing TAT is incubated with antibodies against thrombin, and the unbound constituents are removed by washing. Enzyme conjugated antibodies to antithrombin are then added to the reaction, and the excess antibodies are removed by washing. The remaining (bound) enzymatic activity acts upon a chromophore and color development is proportional to the TAT in the sample.
TAT From Set-Up	3 - 9 Days

Result Code	Result Name	LOINC
500013	Thrombin Antithrombin Complex	14182-0