Test Detail

MGMT (O(6)-Methylguanine-DNA Methyltransferase) Gene Methylation Assay

Test Code:

489280

Performing Lab: LABCORP RTP

CPT Code:

81287 88381

Order Information
Results

Specimen Container	Paraffin Tube
Specimen Type	Glioblastoma specimens in Formalin-fixed, paraffin-embedded (FFPE) tissue blocks or slides.
Volume	FFPE tissue block OR eight unstained slides at 5 uM AND one matching H&E slide OR nine precut unstained slides from a paraffin block in 5-uM sections.
Minimum Volume	50mm2 total tumor area is required for testing. Two unstained slides at 5 uM and one matching H&E slide or three unstained slides at 5 uM.
Temperature	Maintain blocks/slides at room temperature.
Specimen Handling	FFPE tissue block OR eight unstained slides at 5 uM AND one matching H&E slide OR nine precut unstained slides from a paraffin block in 5-uM sections.
Methodology	Quantitative methylation-specific polymerase chain reaction (PCR)
Unacceptable Conditions	Tissue is not primary brain tumor. Tumor block containing insufficient tumor tissue. Tumor fixed in a heavy metal fixative. Broken or stained slides. Decalcified specimens.
TAT From Set-Up	7 - 10 Days

Result Code	Result Name	LOINC
480903	Microdissection Performed	8100-0
489268	Methylation Score:	64083-9
489281	Result:	60252-4
489282	Director Review:	72486-4
489283	Block Number:	UNLOINC