Test Detail

Heparin-dependent Platelet Antibody(Serotonin Release Assay)

Test Code:

150018

Performing Lab: LABCORP BURLINGTON

CPT Code:

82542

Order Information
Results

Specimen Container	Gel-barrier or red top tube
Specimen Type	Serum, FROZEN
Volume	1.0 mL
Minimum Volume	0.5 mL
Temperature	FREEZE
Specimen Handling	Transfer serum to a plastic transport tube. To avoid delays in turnaround time when requesting multiple tests on frozen samples, PLEASE SUBMIT SEPARATE FROZEN SPECIMENS FOR EACH TEST REQUESTED.
Methodology	The SRA employs washed donor platelets and detects their activation by measuring the release of endogenous serotonin induced by addition of patient serum. The serotonin levels are measured by a highly sensitive liquid chromatography/ tandem mass spectroscopy method. Therapeutic levels of heparin included in the assay mix allow for the formation of PF4/heparin complexes on the donor platelet surface to provide an antigen target for the HIT antibodies. The specificity of the assay is increased by assessing the inhibition of platelet activation by adding heparin in excess, thus proving the heparin dependency of the serotonin release.
Unacceptable Conditions	Room temperature specimen received; grossly hemolyzed, icteric, and lipemic samples
Days Set-Up	Monday- Friday
Shift Performed	1st shift
TAT From Set-Up	3 - 7 Days

Result Code	Result Name	LOINC
150014	SRA, Low Dose Heparin	77728-4
150016	SRA, High Dose Heparin	50727-7
150017	SRA, Interpretation	66488-8