Test Detail

Pyruvic Acid, Blood

Test Code:

004788

Performing Lab: LABCORP BURLINGTON

CPT Code:

84210

Order Information
Results

Specimen Container	Gray-top (potassium oxalate/sodium fluoride) tube and one LabCorp 8% perchloric acid tube
Specimen Type	Perchloric acid supernatant from oxalated whole blood
Volume	0.5 mL (Clear supernatant)
Minimum Volume	0.2 mL (Clear supernatant)
Temperature	Maintain the perchloric acid supernatant refrigerated.
Specimen Handling	Draw blood into chilled gray-top tube. Immediately after blood is drawn, add 2 mL of whole blood from the gray-top tube to 2 mL of 8% perchloric acid (PeopleSoft No. 111749) (7 mL of 70% perchloric acid diluted to 100 mL with deionized water). NOTE: Use 1:1 blood (ie, equal parts) to 8% perchloric ratio if less blood is obtained. Shake mixture vigorously for about 30 seconds. Refrigerate for five minutes to ensure complete protein precipitation. Centrifuge 5 to 10 minutes at approximately 1500xg. Immediately transfer the CLEAR SUPERNATANT to a transport tube. Label the transport tube as "SUPERNATANT"and send to the laboratory. NOTE: A second centrifugation of the supernatant may be necessary to obtain a clear protein-free solution.
Methodology	Pyruvate - lactate; spectrophotometry
Stability	Room Temperature: 5 days, Refrigerated: 7 days, Frozen: 7 days
Unacceptable Conditions	Whole blood received; gray-top tube NOT prepared with perchloric acid
Days Set-Up	TU,TH
Shift Performed	3
TAT From Set-Up	2 - 4 Days

Result Code	Result Name	LOINC
004788	Pyruvic Acid, Blood	2905-8